I-HB Idala I-Paradigm Entsha Yesitoreji Sesampula Sebhayoloji kwa-ICL

I-Imperial College London (ICL) ihamba phambili ophenyweni lwesayensi futhi, ngoMnyango Wezokuvikela kanye Nokuvuvukala kanye noMnyango Wesayensi Yobuchopho, ucwaningo lwayo lusukela ku-rheumatology ne-hematology kuya ekuwohlokeni komqondo, isifo sikaParkinson kanye nomdlavuza wobuchopho. Ukuphatha ucwaningo olunjalo oluhlukahlukene kudinga izinsiza zesimanje, ikakhulukazi ukugcinwa kwamasampuli abalulekile ebhayoloji. U-Neil Galloway Phillipps, Umphathi Omkhulu Welebhu kuyo yomibili iminyango, usibonile isidingo sesixazululo esisebenza kahle nesimeme sokugcina i-cryogenic.

Izidingo ze-ICL

1.Umthamo omkhulu, isistimu yokugcina i-nitrogen ewuketshezi

2.Ukwehliswa kokusetshenziswa kwe-nitrogen nezindleko zokusebenza

3.Ukuphepha kwesampula okuthuthukisiwe nokuhambisana nokulawula

4.Ukufinyelela okuphephile nokusebenza kahle kakhudlwana kwabacwaningi

5.Isixazululo esimeme sokusekela imizamo eluhlaza

Izinselele

Umnyango we-ICL we-Immunology ngaphambilini ubuthembele ku-nitrogen eyi-static liquid (LN) eyi-13 ehlukene.₂) amathangi okugcina amasampula esilingo somtholampilo, amaseli esathelayithi namasiko amaseli ayisisekelo. Lolu hlelo oluhlukene lwaludla isikhathi ukulugcina, ludinga ukugadwa njalo nokugcwaliswa kabusha.

“Ukugcwalisa amathangi ayi-13 kuthathe isikhathi esiningi, nokugcina umkhondo wakho konke kwakuya ngokuba nzima,” kuchaza uNeil. “Kube yinselelo yokuhlela izinto, futhi besidinga indlela esebenza kahle kakhulu yokuphatha isitoreji sethu.”

Izindleko zokunakekela amathangi amaningi kwakungenye inkathazo. LN₂ukusetshenziswa kwakuphezulu, kube nesandla ekunyukeni kwezindleko zokusebenza. Ngesikhathi esifanayo, umthelela wemvelo wokulethwa njalo kwe-nitrogen wawungqubuzana nokuzibophezela kwelebhu ekusimameni. “Besisebenzela ukuthola imiklomelo eyahlukene yokusimama, futhi besazi ukuthi ukwehlisa ukusebenzisa kwethu initrogen kungenza umehluko omkhulu,” kuphawula uNeil.

Ukuphepha nokuhambisana nakho bekuyizinto ezibalulekile ezibalulekile. Njengoba amathangi amaningi asabalale ezindaweni ezahlukene, ukulandelela ukufinyelela nokugcina amarekhodi akamuva kwakunzima. “Kubalulekile ukuthi sazi kahle ukuthi ubani othola amasampula, nokuthi yonke into igcinwe ngendlela ehambisana nemithetho yeHuman Tissue Authority (HTA),” kwengeza uNeil. “Uhlelo lwethu oludala alwenzanga lwaba lula lokho.”

Isixazululo

I-ICL isivele inohlu lwemishini evela ku-Haier Biomedical - ehlanganisa isitoreji esibandayo, amakhabethe okuphepha kwezinto eziphilayo, i-CO₂ama-incubator nama-centrifuges – ukuthuthukisa ukwethenjwa kwezixazululo zenkampani.

Ngakho-ke u-Neil nethimba lakhe bathintana ne-Haier Biomedical ukuze basize ukubhekana nalezi zinselelo ezintsha, bafake i-CryoBio 43 LN enamandla amakhulu.₂i-biobank ukuze ihlanganise wonke amathange ayi-13 amile abe uhlelo olulodwa olusebenza kahle kakhulu. Ushintsho belungenazihibe, nethimba lika-Haier eliphethe ukufakwa nokuqeqesha izisebenzi zelebhu. Isistimu entsha ifakwe ku-LN ekhona₂isikhungo esinokulungiswa okuncane kuphela. Njengoba uhlelo olusha selukhona, ukugcinwa kwesampula nokuphatha sekusebenze kahle kakhulu. “Okunye okuhle obekungalindelekile ukuthi singakanani isikhala esisitholile,” kuphawula uNeil. “Ngawo wonke lawo mathangi amadala asusiwe, manje sinendawo eyengeziwe kulebhu yezinye izinto zokusebenza.”

Ukushintshela endaweni yokugcina yesigaba somhwamuko kuthuthukise kokubili ukuphepha kanye nokusebenziseka kalula.

U-Neil nethimba lakhe bathole isistimu isebenziseka kalula, nohlelo lokuqeqeshwa luka-Haier olubavumela ukuthi bangene ngokushesha kubasebenzisi bokugcina.

Isici esingalindelekile kodwa esamukelekayo kwakuyizinyathelo ezizenzakalelayo ezihoxiswayo, ezenza ukufinyelela ithangi kube lula. "Ngamathangi angaphambilini, abacwaningi ngokuvamile kwakudingeka baphakamise izinto ngaphandle lapho selugcwele. Noma ithangi elisha lilide, izinyathelo zisetshenziswa ngokucindezela inkinobho, okwenza ukwengeza noma ukukhipha amasampula kube lula kakhulu ukuphatha," kuphawula uNeil.

Ukugcina amasampula abalulekile

Amasampula agcinwe endaweni ye-ICL ye-cryogenic abalulekile ocwaningweni oluqhubekayo. “Amanye amasampula esiwagcinayo awanakushintshwa ngokuphelele,” kusho uNeil.

“Sikhuluma ngamalungiselelo amangqamuzana amhlophe egazi avela ezifweni ezingavamile, amasampula okuhlolwa komtholampilo, nezinye izinto ezibalulekile ocwaningweni. Lawa masampuli awagcini nje ngokusetshenziswa elebhu; abelwe nabahlanganyeli emhlabeni wonke, okwenza ubuqotho bawo bubaluleke kakhulu. Ukusebenza kwala maseli kuyinto yonke. Uma engagcinwa kahle, ucwaningo alusekelayo lungase lube sengozini. ukuthula kwengqondo. Singahlola iphrofayili yezinga lokushisa nganoma yisiphi isikhathi, futhi uma sike sacwaningwa, singabonisa ngokuzethemba ukuthi yonke into igcinwe ngendlela efanele.”

 Ukwenza ngcono ukusimama nokusebenza kahle kwezindleko

Ukwethulwa kwe-biobank entsha kunciphise kakhulu ukusetshenziswa kwe-nitrogen ewuketshezi kulebhu, kuyinqamule ngokuphindwe kashumi. “Ithangi ngalinye lalawo amadala laliphethe amalitha angaba ngu-125, ngakho ukulihlanganisa kwenze umehluko omkhulu,” kuchaza uNeil. “Manje sisebenzisa ingxenye ethile ye-nitrogen ebesiyenza ngaphambili, futhi lokho kuwukuwina okukhulu kwezezimali nakwezemvelo.”

Ngokulethwa kwe-nitrogen okumbalwa okudingekayo, ukukhishwa kwekhabhoni kwehlisiwe, okusekela izinhloso zokusimama kwelebhu. “Akukhona nje nge-nitrogen ngokwayo,” kwengeza uNeil. “Ukudiliva okuncane kusho ukuthi kunamaloli ambalwa emgwaqeni, bese kusetshenziswa amandla amancane ukukhiqiza i-nitrogen kwasekuqaleni.” Lokhu kuthuthukiswa bekubaluleke kakhulu kangangokuthi i-Imperial ithole imiklomelo yokusimama kuzo zombili i-LEAF kanye ne-My Green Lab ngokuqaphela imizamo yayo.

Isiphetho

I-cryogenic biobank ye-Haier Biomedical iguqule amandla okugcina e-ICL, yathuthukisa ukusebenza kahle, ukuphepha nokusimama kuyilapho yehlisa kakhulu izindleko. Ngokuthobelana okungcono, ukuvikeleka kwesampula okuthuthukisiwe kanye nomthelela oncishisiwe wemvelo, ukuthuthukiswa kube yimpumelelo emangalisayo.

Imiphumela Yephrojekthi

1.LN₂ukusetshenziswa kwehliswe ngo-90%, ukunciphisa izindleko kanye nokukhishwa kwegesi

2.Ukulandelela isampula okusebenza kahle kakhulu nokuhambisana ne-HTA

3.Isitoreji sesigaba se-vapour esiphephile sabacwaningi

4.Umthamo wokugcina okhulisiwe ohlelweni olulodwa

5.Ukuqashelwa ngemiklomelo yokusimama