I-HB Idala Ipharadayimu Entsha Yokugcina Isampula Yezinto Eziphilayo e-ICL

I-Imperial College London (ICL) ihamba phambili ocwaningweni lwesayensi futhi, ngoMnyango We-Immunology and Inflammation kanye noMnyango Wezesayensi Yobuchopho, ucwaningo lwayo luqala ku-rheumatology kanye ne-hematology kuya ku-dementia, isifo sikaParkinson kanye nomdlavuza wobuchopho. Ukuphatha ucwaningo olunjalo oluhlukahlukene kudinga izikhungo zesimanje, ikakhulukazi zokugcina amasampula abalulekile ezinto eziphilayo. UNeil Galloway Phillipps, uMphathi Omkhulu Welebhu kuzo zombili iminyango, waqaphela isidingo sesisombululo sokugcina i-cryogenic esisebenza kahle nesiqhubekayo.

Izidingo ze-ICL

1.Uhlelo lokugcina i-nitrogen olumanzi olunamandla aphezulu noluhlanganisiwe

2.Ukusetshenziswa kwe-nitrogen okuncishisiwe kanye nezindleko zokusebenza

3.Ukuphepha kwesampula okuthuthukisiwe kanye nokuhambisana nomthetho

4.Ukufinyelela okuphephile nokuphumelelayo kwabacwaningi

5.Isixazululo esizinzile sokusekela izinhlelo eziluhlaza

Izinselele

UMnyango Wezokuzivikela Emzimbeni we-ICL wawuthembele ngaphambilini ku-nitrogen ewuketshezi engashintshi engu-13 (LN) ehlukene.₂) amathangi okugcina amasampula okuhlolwa kwemitholampilo, amaseli e-satellite kanye namasiko amaseli ayinhloko. Lolu hlelo oluqhekekile beluthatha isikhathi ukulunakekela, ludinga ukuqapha njalo nokugcwalisa kabusha.

“Ukugcwalisa amathangi angu-13 kwathatha isikhathi esiningi, futhi ukulandelela konke kwakuba nzima kakhulu,” kuchaza uNeil. “Kwakuyinselele yezokuthutha, futhi sasidinga indlela ephumelela kakhudlwana yokuphatha isitoreji sethu.”

Izindleko zokunakekela amathangi amaningi zaziyinkinga enye.₂ukusetshenziswa kwakuphezulu, okwabangela ukwenyuka kwezindleko zokusebenza. Ngesikhathi esifanayo, umthelela wezemvelo wokulethwa kwe-nitrogen njalo wawuphikisana nokuzibophezela kwelebhu ekusimameni. “Besilokhu sisebenzela ukuthola imiklomelo eyahlukene yokusimamisa, futhi sasazi ukuthi ukunciphisa ukusetshenziswa kwethu kwe-nitrogen kuzokwenza umehluko omkhulu,” kuphawula uNeil.

Ukuphepha nokuthobela imithetho nakho kwakuyizinto eziza kuqala. Njengoba amathangi amaningi esakazeke ezindaweni ezahlukene, ukulandelela ukufinyelela nokugcina amarekhodi asesikhathini kwakuyinkimbinkimbi. “Kubalulekile ukuthi sazi kahle ukuthi ubani othola amasampula, nokuthi konke kugcinwe kahle ngokuhambisana nemithethonqubo ye-Human Tissue Authority (HTA),” kusho uNeil. “Uhlelo lwethu oludala aluzange lwenze lokho kwaba lula.”

Isixazululo

I-ICL yayivele inemishini ehlukahlukene evela ku-Haier Biomedical - ehlanganisa indawo yokugcina izinto ezibandayo, amakhabethe okuphepha ezinto eziphilayo, i-CO2₂ama-incubator kanye nama-centrifuge – ukuthuthukisa ukwethembana ezixazululweni zenkampani.

Ngakho-ke uNeil nethimba lakhe bathintane neHaier Biomedical ukuze basize ekuxazululeni lezi zinselele ezintsha, bafaka i-CryoBio 43 LN enamandla amakhulu.₂i-biobank ukuhlanganisa wonke amathangi ayi-13 angaguquki abe uhlelo olulodwa olusebenza kahle kakhulu. Ushintsho belungenazihibe, ithimba likaHaier liphethe ukufakwa nokuqeqeshwa kwabasebenzi belebhu. Uhlelo olusha lungene ku-LN ekhona₂indawo enezinguquko ezincane kuphela. Njengoba uhlelo olusha selukhona, ukugcinwa kwamasampula kanye nokuphathwa kwawo sekusebenze kahle kakhulu. “Enye yezinzuzo ezingalindelekile ukuthi sithole isikhala esingakanani,” kuphawula uNeil. “Njengoba wonke lawo matanki amadala esusiwe, manje sesinendawo eyengeziwe elabhorethri yeminye imishini.”

Ukushintshela kwisitoreji sesigaba somhwamuko kuthuthukise kokubili ukuphepha kanye nokulula kokusetshenziswa. "Ngaphambilini, njalo lapho sikhipha i-rack ethangini lesigaba soketshezi, lalizoconsa i-nitrogen, okwakuyinkinga yokuphepha njalo. Manje, ngesitoreji sesigaba somhwamuko, kuhlanzekile kakhulu futhi kuphephile ukuphatha amasampula. Uhlelo lokufinyelela lwe-biometric luqinise nokuphepha kanye nokuthobela imithetho ngoba singakwazi ukulandelela ngokunembile ukuthi ubani ofinyelela ohlelweni nokuthi nini."

UNeil nethimba lakhe bathole ukuthi uhlelo lulula ukulusebenzisa, njengoba uhlelo lokuqeqesha lukaHaier lubenza bakwazi ukwamukela abasebenzisi ngokushesha.

Isici esingalindelekile kodwa esamukelekile kwakuyizinyathelo ezikwazi ukuhoxiswa ngokuzenzakalela, ezenza ukufinyelela ethangini kube lula. “Ngamathangi angaphambilini, abacwaningi babevame ukukhuphula izinto ziphume ziphelele. Ngisho noma ithangi elisha lide, izinyathelo zisebenza ngokucindezela inkinobho, okwenza ukwengeza noma ukususa amasampula kube lula kakhulu ukuwaphatha,” kusho uNeil.

Ukulondoloza amasampula abalulekile

Amasampula agcinwe esikhungweni se-ICL esibangela i-cryogenic abaluleke kakhulu ocwaningweni oluqhubekayo. “Amanye amasampula esiwagcinayo awanakushintshwa nhlobo,” kusho uNeil.

"Sikhuluma ngamalungiselelo amangqamuzana amhlophe egazi avela ezifweni ezingavamile, amasampula okuhlolwa kwezokwelapha, nezinye izinto ezibalulekile ocwaningweni. Lawa masampula awasetshenziswa nje kuphela ngaphakathi kwelebhu; abelwana ngawo nabalingani emhlabeni wonke, okwenza ubuqotho bawo bubaluleke kakhulu. Ukuphila kwalawa maseli kuyikho konke. Uma engagcinwanga kahle, ucwaningo alusekelayo lungase lube sengozini. Yingakho sidinga indawo yokugcina ebandayo ethembekile kakhulu esingayethemba. Ngohlelo lwe-Haier, sinokuthula kwengqondo okuphelele. Singahlola iphrofayili yokushisa nganoma yisiphi isikhathi, futhi uma sike sahlolwa, singabonisa ngokuqiniseka ukuthi konke kugcinwe kahle."

 Ukuthuthukisa ukusimama kanye nokusebenza kahle kwezindleko

Ukwethulwa kwe-biobank entsha kunciphise kakhulu ukusetshenziswa kwe-nitrogen ewuketshezi kwelebhu, kwanciphisa ngokuphindwe kayishumi. “Ithangi ngalinye lalawo madala laliphethe amalitha angaba ngu-125, ngakho ukuwahlanganisa kwenze umehluko omkhulu,” kuchaza uNeil. “Manje sisebenzisa ingxenye encane ye-nitrogen esasiyisebenzisa ngaphambili, futhi lokho kuyinzuzo enkulu kokubili ngokwezimali nangokwezemvelo.”

Njengoba kudingeka ukulethwa kwe-nitrogen encane, ukukhishwa kwekhabhoni kuye kwehla, okusekela imigomo yokuzinza kwelebhu. “Akukhona nje nge-nitrogen uqobo,” kusho uNeil. “Ukuba nokulethwa okuncane kusho amaloli ambalwa emgwaqweni, kanye namandla amancane asetshenziswa ukukhiqiza i-nitrogen kwasekuqaleni.” Lokhu kuthuthuka kwakubaluleke kakhulu kangangokuthi i-Imperial yathola imiklomelo yokuzinza evela ku-LEAF kanye ne-My Green Lab ngokubona imizamo yayo.

Isiphetho

I-cryogenic biobank ye-Haier Biomedical iguqule amakhono okugcina e-ICL, yathuthukisa ukusebenza kahle, ukuphepha kanye nokusimama ngenkathi inciphisa kakhulu izindleko. Ngokuhambisana nomthetho okungcono, ukuvikeleka kwesampula okuthuthukisiwe kanye nomthelela oncishisiwe emvelweni, ukuthuthukiswa kube yimpumelelo enkulu.

Imiphumela Yephrojekthi

1.LN₂ukusetshenziswa kwehliswe ngo-90%, okunciphisa izindleko kanye nokukhishwa kwegesi

2.Ukulandelela amasampula okusebenza kahle kakhulu kanye nokuhambisana ne-HTA

3.Isitoreji esiphephile sesigaba somusi sabacwaningi

4.Umthamo wesitoreji owandisiwe ohlelweni olulodwa

5.Ukuqashelwa ngemiklomelo yokuzinza