Amathangi e-nitrogen asetshenziswa kabanzi emikhakheni ye-biomedicine, isayensi yezolimo kanye nezimboni.Lawa mathangi angasetshenziswa ngezindlela ezimbili: ukugcinwa kwesigaba somhwamuko nokugcinwa kwesigaba soketshezi, ngayinye inezinzuzo zayo eziyingqayizivele kanye nokubi.
I. Ubuhle nokubi kokugcinwa kwesigaba somhwamuko emathangeni e-nitrogen ewuketshezi:
Ukugcinwa kwesigaba somhwamuko kuhilela ukuguqula i-nitrogen ewuketshezi ibe isimo esinegesi egcinwe ngaphakathi kwethangi.
Izinzuzo:
a.Ukufaneleka: Ukugcinwa kwesigaba somhwamuko kuqeda ukukhathazeka mayelana nokuhwamuka nokulawulwa kwezinga lokushisa kwe-nitrogen ewuketshezi, okwenza umsebenzi ube lula futhi ube lula.
b.Ukuphepha: Njengoba i-nitrogen ewuketshezi isesimweni segesi, ingozi yokuvuza koketshezi iyancishiswa, okuthuthukisa ukuphepha.
c.Ukuhlukahluka: Ukugcinwa kwesigaba somhwamuko kulungele ukugcina inani elikhulu lamasampula, njengamasampula ebhayoloji kanye nembewu yezolimo.
Ububi:
a.Ukulahleka kokuhwamuka: Ngenxa yezinga eliphezulu lokuhwamuka kwe-nitrogen ewuketshezi, ukugcinwa kwesigaba somhwamuko eside kungaholela ekulahlekeni kwe-nitrogen, kukhuphule izindleko zokusebenza.
b.Isikhathi esilinganiselwe sokulondoloza: Uma siqhathaniswa nesigaba sokugcina uketshezi, indawo yokugcina umhwamuko inesikhathi esifushane sokulondoloza isampula.
II.Izinzuzo kanye nobubi bokugcinwa kwesigaba se-liquid emathangini e-nitrogen ewuketshezi:
Ukugcinwa kwesigaba soketshezi kubandakanya ukugcina ngokuqondile i-nitrogen ewuketshezi ethangini.
Izinzuzo:
a.Isitoreji esiminyana kakhulu: Isitoreji sesigaba soketshezi singagcina umthamo omkhulu we-nitrogen ewuketshezi endaweni encane, okwandisa ukuminyana kwesitoreji.
b.Ukulondolozwa kwesikhathi eside: Uma kuqhathaniswa nokugcinwa kwesigaba somhwamuko, ukugcinwa kwesigaba soketshezi kungagcina amasampula isikhathi eside, kunciphise ukulahleka kwesampula.
c.Izindleko zokulondoloza eziphansi: Isitoreji sesigaba soketshezi sibiza kakhulu uma siqhathaniswa nokugcinwa kwesigaba somhwamuko.
Ububi:
a.Ukulawula izinga lokushisa: Ukulawulwa kwezinga lokushisa okuqinile kuyadingeka ukuze kugcinwe isigaba soketshezi ukuze kuvinjelwe ukuhwamuka okukhulu kanye nokubanda kwesampula.
b.Izingozi zokuphepha: Ukugcinwa kwesigaba soketshezi kuhlanganisa ukuthintana ngokuqondile ne-nitrogen ewuketshezi, okudala izingozi zokuvuza kwe-nitrogen nokusha, okudinga ukunakwa okukhethekile ezinqubweni zokuphepha.
III.Izicelo zesigaba se-liquid kanye nesigaba somhwamuko:
Isigaba soketshezi kanye nesigaba sokugcina somoya sisebenzisa izinjongo ezihlukene ezinhlelweni ezihlukahlukene.
Izinhlelo zokusebenza zesitoreji sesigaba se-liquid:
a.I-Biomedicine: Isitoreji sesigaba samanzi sisetshenziswa kakhulu ku-biomedicine ukuze kulondolozwe amasampula ezinto eziphilayo, amaseli, izicubu, njll., ukusekela ucwaningo lwezokwelapha kanye nokuxilongwa.
b.Ibhayoloji yezoLimo: Ososayensi bezolimo basebenzisa isitoreji sesigaba soketshezi ukuze balondoloze imbewu ebalulekile, impova, nemibungu eqandisiwe, bavikele izinsiza zofuzo zezitshalo nokuthuthukisa izinhlobo.
c.Isitoreji somuthi wokugoma: Ukugcinwa kwesigaba soketshezi kuyindlela evamile yokugcina imithi yokugoma, eqinisekisa ukuzinza nokusebenza kwayo kwesikhathi eside.
d.I-Biotechnology: Ku-biotechnology, ukugcinwa kwesigaba soketshezi kusetshenziselwa ukulondoloza amabhange ezakhi zofuzo, ama-enzyme, amasosha omzimba, namanye ama-reagents ebhayoloji abalulekile.
Izinhlelo zokusebenza zokugcinwa kwesigaba somhwamuko:
a.Amalabhorethri amasiko amaseli: Kulabhorethri ye-cell culture, ukugcinwa kwesigaba somhwamuko kulungele ukugcinwa kwesikhashana kwemigqa yamaseli kanye namasiko amaseli.
b.Isitoreji sesampula sesikhashana: Kumasampuli esikhashana noma lawo angadingi ukulondolozwa isikhathi eside, indawo yokugcina umhwamuko ihlinzeka ngesixazululo esisheshayo nesilula sokulondoloza.
c.Ukuhlolwa okunezidingo eziphansi zesiqandisi: Ngokuhlolwa okunezidingo zesiqandisi ezingaqinile, ukugcinwa kwesigaba somhwamuko kuwukukhetha ukonga kakhulu.
Amathangi e-nitrogen ewuketshezi anesigaba somhwamuko nendawo yokugcina uketshezi ngalinye linobuhle nobubi balo.Ukukhetha phakathi kwezindlela zokugcina kuncike ezimweni ezithile zohlelo lokusebenza kanye nezidingo.Isitoreji sesigaba soketshezi sifanele isitoreji sesikhathi eside, isitoreji esiminyana kakhulu, kanye nezimo ezinezidingo eziphakeme zezomnotho.Ngakolunye uhlangothi, ukugcinwa kwesigaba se-vapor kulula kakhulu, kulungele ukugcinwa kwesikhashana kanye nezimo ezinezidingo eziphansi zesiqandisi.Kuzinhlelo zokusebenza ezingokoqobo, ukukhetha indlela efanele yokugcina ngokusekelwe ezicini zesampula nezidingo zokulondoloza kuzofaka isandla ekuthuthukiseni ukusebenza kahle komsebenzi kanye nekhwalithi yesampula.
Isikhathi sokuthumela: Dec-10-2023
