Emikhakheni yebhayoloji kanye nezokwelapha, ukuvikelwa kwamasampula ebhayoloji kubaluleke kakhulu. Ngaphandle kokuthi "ulele" ezindaweni zokucwaninga nasezibhedlela, lawa masampula avame ukudinga ukuthuthwa. Ukuze kugcinwe noma kuthuthwe ngokuphephile lawa masampula ayigugu ebhayoloji, ukusetshenziswa kwamathangi e-nitrogen ewuketshezi emazingeni okushisa aphansi kakhulu angama-degree Celsius angu-196 kubaluleke kakhulu.
Amathangi e-nitrogen angoketshezingokuvamile ahlukaniswa ngezinhlobo ezimbili: amathangi okugcina i-nitrogen ewuketshezi kanye namathangi okuthutha i-nitrogen ewuketshezi. Amathangi okugcina asetshenziswa kakhulu ukulondolozwa kwe-nitrogen ewuketshezi ngaphakathi endlini, enamandla amakhulu kanye nobuningi obungafaneleki kangako ukuthuthwa ibanga elide ezimweni zokusebenza.
Ngokuphambene nalokho, amathangi okuthutha ane-nitrogen ewuketshezi alula kakhulu futhi aklanyelwe ukuhlangabezana nezidingo zokuhamba. Ukuqinisekisa ukuthi afanelekile ukuthuthwa, lawa matanki aklanywa ngendlela ekhethekile yokulwa nokudlidliza. Ngaphandle kokugcinwa okungaguquki, angasetshenziswa ukuthuthwa ngenkathi egcwele i-nitrogen ewuketshezi, kodwa kumele kuthathwe izinyathelo zokuphepha ukuze kugwenywe ukushayisana okukhulu nokudlidliza.
Isibonelo, i-Liquid Nitrogen Biobanking Series ye-Haier Biomedical iyakwazi ukuthutha amasampula ezinto eziphilayo ezindaweni ezijulile zokushisa eziphansi kakhulu. Ukwakheka kwayo kuvimbela ngempumelelo ukukhishwa kwe-nitrogen ewuketshezi ngesikhathi sokuthuthwa.
Ezimweni lapho abasebenzi bedinga ukuthuthwa ngendiza isikhathi esifushane, i-Biobanking Series ibaluleke kakhulu. Lolu chungechunge lunesakhiwo se-aluminium esiqinile esinezincazelo ezinhlanu zamavolumu ongakhetha kuzo, iwaranti ye-vacuum yeminyaka emi-3, eqinisekisa ukuphepha kwesikhathi eside kwamasampula. Amathangi angagcina ama-cryogenic vials noma amashubhu okuqandisa ajwayelekile angu-2ml, afakwe isihlukanisi se-mesh sensimbi engagqwali esikhethekile sendawo yokugcina kanye nomzimba wokufaka i-nitrogen ewuketshezi. Izivalo ezingakhiywa ezingakhethwa zengeza ungqimba olwengeziwe lokuphepha endaweni yokugcina amasampula.
Nakuba ukwakheka kwamathangi e-nitrogen ewuketshezi kwenza kube lula ukuthutha, kumele kulandelwe izinyathelo zokuphepha eziningana kuyo yonke inqubo yokuthutha. Okokuqala, kubalulekile ukuqinisekisa ukuthi wonke ama-valve switch ethangini le-nitrogen ewuketshezi asesimweni esifanayo nesikhathi sokugcina. Ngaphezu kwalokho, ithangi kufanele lifakwe ngaphakathi kohlaka lokhuni olune-cushion efanele, futhi uma kudingeka, liboshwe emotweni yokuthutha kusetshenziswa izintambo ukuvimbela noma yikuphi ukunyakaza ngesikhathi sokuthutha.
Ngaphezu kwalokho, ukusebenzisa ama-filler phakathi kwamatangi kubalulekile ukuvimbela ukushayisana kanye nemithelela ngesikhathi sokuthuthwa, ngaleyo ndlela kugwenywe izingozi. Lapho ulayisha futhi uthulula amathangi e-nitrogen ewuketshezi, kufanele kuqashelwe ukuvimbela ukuthi angashayisani. Ukuwahudula phansi akukhuthazwa kakhulu, njengoba kunganciphisa isikhathi sokuphila kwamatangi e-nitrogen ewuketshezi.
Isikhathi sokuthunyelwe: Jan-04-2024
