Eminyakeni yamuva nje, ama-biobanks abelokhu edlala indima ebaluleke kakhulu ocwaningweni lwesayensi.Imishini yokugcina izinga lokushisa eliphansi yekhwalithi ephezulu ingaqinisekisa ukuphepha nomsebenzi wamasampuli futhi isize abacwaningi ekwenzeni kangcono ucwaningo oluhlukahlukene lwesayensi ngokuhlinzeka ngendawo yokugcina efanele nephephile yamasampuli ebhayoloji.
Amathangi e-nitrogen ewuketshezi asetshenziselwe ukugcina amasampula isikhathi eside.Bagcina amasampula ezingeni lokushisa eliphansi elingu -196 ℃ elidalwe ngesisekelo somgomo wokuvala i-vacuum ngemva kokuba amasampula esepholiswe ngaphambili.Kunezindlela ezimbili zamathangi e-nitrogen ewuketshezi ukugcina amasampula: ukugcinwa kwesigaba soketshezi kanye nokugcinwa kwesigaba somhwamuko.Uyini umehluko phakathi kwalokhu okubili?
1. Isicelo
Amathangi e-nitrogen esigaba samanzi asetshenziswa kakhulu kumalabhorethri, ukufuywa kwezilwane, kanye nomkhakha wokucubungula.
Amathangi e-nitrogen liquid wesigaba se-vapor asetshenziswa kakhulu kuma-biobanks, kwezokwelapha, kanye nenkundla yokunakekelwa kwezempilo.
2. Isimo Sesitoreji
Esigabeni somhwamuko, amasampula agcinwa ngokuhwamuka kanye nokupholisa initrogen eyiketshezi.Izinga lokushisa lesitoreji lisukela phezulu kuye phansi endaweni yokugcina isampula.Uma kuqhathaniswa, esigabeni soketshezi, amasampula agcinwa ngokuqondile ku-nitrogen ewuketshezi ku--196 °C.Amasampula kufanele acwiliswe ngokuphelele ku-nitrogen ewuketshezi.
I-Haier Biomedical Liquid Nitrogen Container-Smart Series
Ngaphezu kwalomehluko, amazinga okuhwamuka kwe-nitrogen ewuketshezi kokubili nawo ahlukile.Ngokuvamile, izinga lokuhwamuka kwe-nitrogen ewuketshezi lingaphansi kobubanzi bethangi le-nitrogen eliwuketshezi, imvamisa yabasebenzisi abavula isivalo, inqubo yokukhiqiza, ngisho nezinga lokushisa elizungezile kanye nomswakama.Kodwa ngokwemvelo, ubuchwepheshe obuthuthukisiwe be-vacuum kanye ne-insulation esetshenziswa ekukhiqizeni amathangi e-nitrogen ewuketshezi kuwukhiye wokuqinisekisa ukusetshenziswa okuphansi kwe-nitrogen ewuketshezi.
Umehluko omkhulu phakathi kwakho kokubili usendleleni amasampula agcinwa ngayo.Egcinwe esigabeni somhwamuko, amasampula awathinti ngokuqondile i-nitrogen ewuketshezi, ukuvimbela amagciwane ukuthi angcolise amasampula.Nokho, izinga lokushisa lokulondoloza alikwazi ukufinyelela -196°C.Esigabeni se-liquid, nakuba amasampula angagcinwa cishe ku--196 °C, ishubhu ye-cryopreservation ayizinzile.Uma ishubhu le-cryopreservation lingavalwanga kahle, i-nitrogen eyiketshezi izongena eshubhu.Lapho ishubhu lokuhlola likhishwa, ukushibilika kwe-nitrogen ewuketshezi kuzoholela ekucindezelekeni okungalingani ngaphakathi nangaphandle kweshubhu lokuhlola futhi ishubhu lizoqhuma ngenxa yalokho.Ngakho-ke, ubuqotho besampula buzolahleka.Lokhu kusikisela ukuthi kukhona kokubili okuhle nokubi endleleni ngayinye.
Ungashaya Kanjani Ibhalansi Phakathi Kwalokhu Okubili?
Uchungechunge lwe-biobank lwe-Haier Biomedical Liquid Nitrogen Storage System luklanyelwe kokubili ukugcinwa kwesigaba soketshezi kanye nomhwamuko.
Ihlanganisa izinzuzo zako kokubili ukugcinwa kwesigaba somhwamuko nokugcinwa kwesigaba soketshezi, okuklanywe ngobuchwepheshe be-vacuum obuthuthukisiwe kanye nokufakwa kwezinto ukuze kuqinisekiswe ukuphepha kwesitoreji kanye nokufana kwezinga lokushisa kuyilapho kunciphisa ukusetshenziswa kwe-nitrogen ewuketshezi.Umehluko wokushisa wayo yonke indawo yokugcina aweqi ku-10°C.Ngisho nasesigabeni somhwamuko, izinga lokushisa lokugcina eduze nendawo ephezulu yeshalofu liphansi ngokuthi -190°C.
I-Biobank Series Yesitoreji Esikhulu
Ukwengeza, izinga lokushisa elinemba okuphezulu nezinzwa zezinga le-liquid zisetshenziselwa ukuqinisekisa ukunemba.Yonke idatha namasampuli avikelwe uhlelo lokulawula ukufinyelela oluvikelekile.Lezi zinzwa ziqapha izinga lokushisa kanye nolwazi lwezinga loketshezi ethangini le-nitrogen eliwuketshezi ngesikhathi sangempela, ngakho-ke uketshezi ethangini lungagcwaliswa ngokuzenzakalelayo ukuze kudalwe izimo zokugcina isampula eziphephe kakhulu.
Isikhathi sokuthumela: Feb-26-2024
